Mass spectrometry based proteomics

Mass spectrometry based proteomics by Indian Institute of Technology Bombay

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Created by IIT Bombay Staff Last updated Wed, 30-Mar-2022 English


Mass spectrometry based proteomics free videos and free material uploaded by IIT Bombay Staff .

Syllabus / What will i learn?

Week 1: Proteomics introduction and sample preparation

Introduction to proteomics

Why proteomics and sample preparation

Protein extraction

In-gel & in-solution digestionLec 5: Fundamentals of mass spectrometry

Week 2: Basics of mass spectrometry

Chromatography technologies

Liquid chromatography

Mass spectrometry: Ionization sources

Mass spectrometry: Mass analyzers

MALDI sample preparation and analysis

Week 3: Quantitative proteomics

Introduction to quantitative proteomics

Hybrid mass spectrometry configurations

SILAC: In vivo labeling

iTRAQ: In vitro labeling

TMT: In vitro labeling

Week 4: Proteomics and systems biology

Quantitative proteomics data analysis

Proteomics and systems biology-I

Proteomics and systems biology-II

Proteomics applications

Challenges in proteomics



Curriculum for this course
0 Lessons 00:00:00 Hours
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Description

Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio. MALDI and ESI- coupled with mass analyzers are commonly used mass spectrometer configuration in proteomics. The human proteome draft was decoded by using high-resolution liquid chromatography coupled with mass spectrometry.

In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.

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