NMR spectroscopy for Chemists and Biologists

Week 1 :

Nuclear Spin and Magnetic Moments

Nuclear Spins in a Magnetic Field Spin

Lattice Relaxation Spin temperature Resonance

Absorption of Energyand The NMR Experiment Resonance

Absorption of Energy and The NMR Experiment Kinetics of Resonance Absorption

Week 2 :

Selection Rules and Line widths

Bloch equations

More about relaxation

More about relaxation Sensitivity

Week 3 :

Instruction to operator Algebra

Chemical Shift

Anisotropy ofchemical shifts Learning spectral simulation

Week 4 :

Factors Influencing Isotropic Chemical shifts: Spin Spin Coupling

Analysis of NMR spectra of molecules

Learning spectral simulation

Week 5 :

Dynamic Effects in the NMR spectra : Two site exchange

Collapse of spin multiplets

Conformational Averaging of J- values

Analysis of NMR spectra of molecules with J Values

Week 6 :

Principles of Fourier transform

NMR Theorems on Fourier transforms

Practical aspects of recording FTNMR spectra

Free Induction Decay (FID) and the spectrum

Pulse repetition rate

Folding of signals

Acquisition time and the resolution

Data processing in FT

NMR Learning of Data processing

Week 7 :

Dynamic range in FTNMR and solvent suppression

The Nuclear Overhauser Effect - Experimental Schemes,Advanced Treatment

Steady state NOE and Transient NOE

Week 8 :

Spin Echo Uncoupled spins

Spin Echo Coupled spins

Spin-lattice relaxation

Spin-spin relaxation

Polarization transfer SPT and INEPT spectralsimulation

Week 9 :

Density matrix, Elements of Density Matrix, Time evolution of density operator

Time evolution of density operator

Product operatorformalism

Week 10 :

Segmentation of the time axis

Two dimensional NMR 2D

Fourier Transformation in NMR

Peak shapes in 2D spectrum

Quadrature detection in two-dimensional NMR

Week 11 :

2D- resolution/ separation experiments

Two-dimensional correlation experiments COSY,TDQ-COSY etc TOCSY

Week 12 :

2D NOESY, 2D ROESY, Heteronuclear COSY,

The HETCOR pulse sequence HSQC