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Week 1:
Introduction to interactomics
An overview of label-free technologies
An overview of surface plasmon resonance (SPR)
An overview of surface plasmon resonance imaging
Basics of SPR: Surface chemistry
Week 2:
Basics of SPR: Experimental design
Protein immobilization for protein-protein interaction studies
Protein-protein interaction study: Binding analysis
Protein-protein interaction study: Kinetic analysis
Protein-small molecule interaction study: Immobilization & binding analysis
Week 3:
Protein-small molecule interaction study: Kinetic analysis
SPR: Interactive Session-I
SPR: Interactive Session-II
An overview of ellipsometry and interferometry techniques
An introduction to BioLayer Interferometry (BLI) and its applications in protein research
Week 4:
Kinetic analysis of protein-protein interaction using BLI
Label-free quantification of proteins using BLI
Diffraction-based biosensors I
Diffraction-based biosensors II
Nanotechniques in proteomics I
Nanotechniques in proteomics II
Week 5:
High throughputplatforms of interactomics: Protein arrays
Conventional label based detection techniques for Protein microarrays
Novel detection techniques for Protein microarrays
Recombinational cloning and its application for Protein microarrays
An introduction to Cell-free protein synthesis
Week 6:
Cell-free synthesis based protein microarrays: PISA and NAPPA Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
Digging deeper into NAPPA: Basic Workflow
Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
Application of cell free expression protein microarrays in biomarker discovery
Week 7:
Application of cell free expression protein microarrays in immunological studies
Basics of microarray image scanning
Software for Image scanning and data processing
Microarray Data Analysis: Part I
Microarray Data Analysis: Part II
Week 8:
Application of protein microarray in biomarker discovery-I
Application of protein microarray in biomarker discovery-II
Systems biology and networks
Challenges in proteomics
Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.
This course of interactomics focuses on two powerful platforms:
1. Protein microarrays and
2. Label free biosensors involving Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI).
While the former is a high-throughput tool for discovery-based interactomics, the later is proving itself to be an instrumental resource for validation based studies. This course details the overlapping domains of proteomics and interactomics, which form the focus of researchers making an impact in the clinical biomarker and pharmaceutical arena across the globe. Students would benefit hugely from this unique course-model designed for budding scientists as well as seasoned researchers new to this area of research
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